:::::;:;::::::;::::::::::::;:::;:;:;:::;:::::: :;:::::.::;:::;::::;:::::;::;:;:;:;:;:::}:;:;:;:;:::;:;:;:::::::;::::::.:::.:.:.: .. : .. : : .Volume 17 Number 3A SPECIAL MESSAGETO MEDICAL ALUMNIIn my inaugural address I found occasion to use the Schoolof Medicine of the University of Chicago as an example of aprofessional function within a university that is not a drain onbut an asset to the total university enterprise.The more I do my homework on the activities of the Divisionof Biological Sciences, the more astonished I become at therange and depth of the Division's activities. The program fortraining medical doctors shows the rigorous thought that hasgone into keeping the University of Chicago School of Medicineamong the nation's top-ranking institutions. This position ofeminence gives the University of Chicago a special set of re­sponsibilities for the nationwide concern of maintaining thequality of students entering upon a medical career. We wantthe next generation of doctors to be as good as-or even betterthan-you are. This is a task in which we will need your help inmany ways.I arrived at the University of Chicago after Dean Bennettcame here from the University of Washington. He is an out­standing authority in the biological field, and both the Divisionand the School of Medicine will continue in the pioneer spiritunder his leadership. As you know, Dr. Coggeshall has movedfrom Billings Hospital to the fifth floor of the AdministrationBuilding and his wisdom has been invaluable to me as I takeover this new office of mine.One of my strongest impressions in getting to know the Di­vision of Biological Sciences resolves itself into one word-themedical term, symbiosis. This concept has brought together notonly some of the world's authorities in their special field butpediatricians who are experts in sociology, surgeons who per­form electronic marvels and computer-oriented cardiologists.The School was based on the belief that only through theclosest association of the clinical and pre-clinical scienceswould there be medical progress. We long since have movedfrom this original relationship into an integration that focusesthe physical as well as the biological sciences into the researchand clinical areas.Looking back over the clinical insights that have arisen outof my own field of study, I believe that the future of medicineat the University of Chicago lies in continuing and strengthen­ing this distinctive tradition of the School of Medicine.GEORGE WELLS BEADLE, ChancellorFrom the walls of Green Hall acrossUniversity Avenue from the Chancellor'sHouse, this welcome greeted the Beadleson the morning of inauguration.MEDICAL ALUMNI BULLETIN 3FACULTY NEWS IWilliam E. Adams has been appointed byGovernor Kerner to the Advisory Commit­tee on Diseases of the Chest of the WelfareDepartment of the State of Illinois.Wright Adams was named chairman ofthe American Board of Internal Medicine.H. Stanley Bennett, Charles Huggins,and H. Burr Steinbach have been named tothe twenty-four member President's Confer­ence on Heart Disease and Cancer underthe chairmanship of Boisfeuillet Jones, spe­cial assistant to the secretary of Health,Education, and Welfare.Lowell T. Coggeshall has been electedto the Board of Directors of CommonwealthEdison Company and of Abbott Labora­tories.James Crawford, '54, instructor in medi­cal psychology, was granted the Ph.D. de­gree in biopsychology in March.Frank W. Fitch, '53, has been selected aMarkle Scholar for 1961-66. He is assistantprofessor of pathology.H. Close Hesseltine was presented witha certificate of appreciation by the Sanga­mon County Medical Society for his "de­voted interest, outstanding leadership anduntiring services as president of the IllinoisState Medical Society."Charles Huggins was awarded the high­est honor of the Peruvian government. Pres­ident Manuel Pardo made the presentationof the Order of the Sun, Class Grand Officer,to Dr. Huggins at the presidential palace inLima.Nathaniel Kleitman gave the annualMurray B. Gordon Lecture at the NewYork Downstate Medical Center in Brook­lyn. His subject was "The 24-Hour Rhythmin Man."The estate of Fred Conrad Koch hasbeen bequeathed to the Endocrine Societyto establish an honorarium of $3,500 to begiven annually with a medal to an individualfor work of special distinction in endocrinol­ogy. Nominations must be made by mem­bers of the society and are limited to citi­zens of the United States and Canada. Pro­fessor Koch was a pioneer in the isolation ofthe androgens. He was Distinguished ServiceProfessor and Chairman of the Departmentof Biochemistry.Joyce Lashof has been appointed fuII­time director of the section of preventivemedicine at Presbyterian-St. Luke's Hos­pital. She will be in charge of clinical medicallaboratories.Henry T. Ricketts has been elected adirector of the Chicago Diabetes Associationfor a three-year term.Henry L. Wild berger, '51, assistant pro­fessor of medicine, has been elected to theBoard of Directors of the Chicago DiabetesAssociation.F. Howell Wright has resigned as chair­man of the Department of Pediatrics. Hewill continue as professor and will devotehimself to research, clinical work, and teach­ing.Dr. Wright's successor has not been ap­Pointed. WRIGHT ADAMS NAMEDASSOCIATE DEANDean H. Stanley Bennett announcedthe appointment on April 15 of WrightRowe Adams as Associate Dean of theBiological Sciences, Dean of the Clini­cal Faculty, and Chief of Staff of TheUniversity of Chicago Clinics.Dr. Adams has been chairman of theDepartment of Medicine since 1949. Inannouncing his new appointment, Dr.Bennett said, "He will have broad re­sponsibilities in the general areas relat­ing to clinical teaching, internships andresidencies, patient care, and profes­sional relationships. His fairness, hisperceptive insight into human relations,his long experience in academic medi­cine, and his outstanding abilities as a clinician and as a teacher will be in­valuable in his new post."Dr. Bennett has asked these profes­sors to advise him in the search for anew permanent chairman of the Depart­ment of Medicine: Cornelius Vermeu­len, '37, Professor of Urology, RobertW. Wissler, '49, Professor of Pathology,William R. Barclay, Associate Profes­sor of Medicine, H. Burr Steinbach,Professor of Zoology, Herbert DanielLandahl, Professor of MathematicalBiology, .and Wright Adams.Leon O. Jacobson, '39, director ofthe Argonne Cancer Research Hospital,will be acting chairman until Dr.Adams' successor is named.4 MEDICAL ALUMNI BULLETINTHE SENIOlALKON, ELLEN BOGENBorn Apr. 10,1936; Stanford University, A.B., 1955;Intern.: Billings; Specialty undeterminded; Married;5715 S. Drexel Ave., Chicago 37; Arcadia, Calif.ANAGNOSTOPOULOS, LAMPIS DEMETRIOSBorn Mar. 29,1930; U. of Chicago, S.B., 1957; In­tern.: Philadelphia General H.; Internal Medicine; Un­married; % Psyhogios, 952 Addison St., Chicago 13.ANDERSON, DAVID GOTFRIEDBorn Sept. 20, 1935; St. Olaf College, Northfield,Minn., A.B., 1957; Intern.: Rockford Memorial H.JRockford, Ill.; Pathology or pediatrics; Unmarried;1825 Parkview Ave., Rockford, Ill.ANDERSON, ROBERT LEEBorn June 28, 1935; Purdue U., S.B., 1957; Intern.:Navy H., Portsmouth, Va.; Obstetrics & Gynecology;Married; Two children; 318 N. Franklin Rd., In­dianapolis, Ind.ATTIG, CHARLES EMERSONBorn July 23, 1929; North Central College, Naper­ville, 111., A.B., 1952; Intern.: Blodgett Memorial H.;Anesthesiology; Married; 438 S. Columbia, Naperville,Ill.AUERBACH, ARTHUR MICHAELBorn Feb., 19,1937; Cornell U., A.B., 1957; Intern.:New York H.; Orthopedic surgery; Unmarried;A.o.A.; 1477 E. 23rd St., Brooklyn, N.Y.BARBER, DAVID HOUGHTONBorn July 21, 1935; Beloit College, S.B., 1957; In­tern.: Cleveland Clinics; General surgery; Married;2020 E. 93rd St., Cleveland 6, Ohio; 194 Sunset, GlenEllyn, Ill.BARBER, NANCY DONNELLBorn Feb. 28,1936; Beloit College, S.B., 1957; In­tern.: Cleveland Clinics; Orthopedic surgery; Married;2020 E. 93rd St., Cleveland 6, Ohio.BASKIND, ROBERTBorn Jan. 23, 1937; U. of Chicago, S.B., 1958; In­tern.: Michael Reese H.; Urology; Unmarried; 3130Lake Shore Dr., Chicago.BEAL, DAVID DIXONBorn June 28,"1935; U. of Washington, S.B., 1957;Intern: U.S.P.H.S. Hosp., Seattle; Surgery; Married;Two children; 803 W. 3rd, Anaconda, Mont.BECKLUND, ROGER WESLEYBorn July 31, 1934; St. Olaf College, Northfield,Minn., A.B., 1957; Intern.: Blodgett Memorial H.;General practice; Married; Almont, N.Dak.BROWNLEE, ALBERT WALTERBorn June 8, 1932; Central State College, Okla.,S.B., 1959; Intern.: Hillcrest Medical Center, Tulsa,Okla.; General practice or obstetrics & gynecology;Married; Two children; 506 E. Vilas, Guthrie, Okla.CAPLAN, ROBERT HENRYBorn Feb. 24,1936; Purdue U., S.B., 1958; Intern.:Highland-Alameda County H., Oakland, Calif.; In­ternal medicine; Unmarried; 2104 S. 56th Ave.,Cicero, Ill. "COE, FRED LAWRENCEBorn Dec. 25, 1936; U. of Chicago, A.B., S.B., 1957;Intern.: Michael Reese H., Internal medicine; Un­married; A.O.A.; 3500 Lake Shore Dr., Chicago.CRAMER, DAVID ALANBorn Nov. 6, 1936; Oberlin College, A.B., 1957;Intern.: Boston City H.; Internal medicine; Unmar-MEDICAL ALUMNI BULLETIN 5;LASS OF 1961DIAMOND, IVAN FREDBorn May 7, 1935; U. of Chicago, A.B., 1956, B.S.,1957; Intern.: New England Center H., Boston; Pedi­atric neurology; Married; A.O.A.; 3093 Brighton 4Street, Brooklyn, N.Y.DUMONT, MATTHEW PIERCEBorn Jan. 29, 1937; Columbia U., A.B., 1957; In­tern.: Boston City H., Psychiatry; Married; 2102Ocean Parkway, Brooklyn, N.Y.EATON, ROBERT PHILIPBorn Sept. 21, 1935; College of Wooster, A.B., 1957;Intern.: Barnes H., St. Louis; Internal medicine; Un­married; A.O.A.; Big Bass Lake, Irons, Mich.ECONOMUS, PETERBorn Feb. 11, 1935; Augustana College, Rock Is­land, III., A.B., 1957; Intern.: Illinois Research &Educational H.; Pediatrics; Unmarried; 256 15th Ave.,East Moline, Ill.ERNEST, JOHN TERRYBorn June 26, 1935; Northwestern U., A.B., 1957;Intern.: Indiana University H., Indianapolis;Ophthalmology; Unmarried; 495 S. Main St., Syca­more, Ill.FALKENBURG, NEIL EDGARBorn June 14, 1931; U. of Chicago, S.B., 1961; In­tern.: U. of Washington H.; Pediatrics; Married; Onechild; 565 McKinley Terrace, Huntington, N.Y.FEIN, JERRY EDWARDBorn Nov. 19, 1934; Morningside College, SiouxCity, Iowa, S.B., 1957; Intern.: Colorado General H.;Internal medicine; Married; One child; A.O.A.; 2314Grandview Blvd., Sioux City 4, Iowa.FILMANOWICZ, EDWARD VINCENT, JR.Born Mar. 28, 1936; Harvard U., A.B., 1957; In­tern.: U. of Michigan H.; Internal medicine; Unmar­ried; 2600 E. 87th St., Chicago 17.FORD, ELIZABETHBorn Apr. 14, 1929; Mount Holyoke College, A.B.,1951, Washington U., M.A., 1957; Intern.: Billings;Specialty undetermined; Unmarried; 654 W. Hill St.,Wabash, Ind.FRIED, FLOYD ALANBorn Mar. 23, 1936; Brooklyn College, S.B., 1957;Intern.: New York H.; Surgery; Unmarried; A.O.A.;825 East Ninth St., Brooklyn 30, N.Y.GEPHART, HARLAN REIDBorn Jan. 26, 1936; Oberlin College, A.B., 1957;Intern.: U. of Iowa H.; Child psychiatry or pediatrics;Married; 151 N. Mozart, Palatine, Ill.GOLDSTONE, DONALD EDWARDBorn May 8, 1936; Johns Hopkins U., A.B., 1957;Intern.: Philadelphia General H.; Specialty undeter­mined; Unmarried j 3400 Ellamont Rd., Baltimore15, Md.GORMLEY, DAVID PAULBorn June 8, 1935; Tufts U., S.B., 1957; Intern.:Philadelphia General H.; Surgery; Unmarried; 31Elder Ave., Bergenfield, N.J.GOTTLIEB, ANTHONY ADAMSBorn Apr. 9, 1936; U. of Illinois; Intern.: Barnes H.,St. Louis; Internal medicine or psychiatry; Unmar­ried; 5543 S. Hyde Park Blvd., Chicago 37.GREEN, JOHN ROBERTBorn Nov. 17, 1931; Harvard U.; Intern.: U. ofWashington H.: Biochemistry; Married; 7817 SouthCL_ ........ T\. .... f"'L! An6 MEDICAL ALUMNI BULLETINTHE SENIORHALL, RICHARD LEEBorn Aug. 6, 1935; Stanford U., A.B., 1957; In­tern.: King County H., Seattle; Surgery; Unmarried;2186 Forty-first Ave., San Francisco, Calif.HALPERIN, DAVID CARLOSBorn May 5, 1937; National U. of Mexico, A.B.,1956; Intern.: Billings; Internal medicine; Unmar­ried; U. of Chicago Clinics.HINMAN, ROBERT CHARLESBorn July 16, 1935; Lawrence College, S.B., 1957;Intern.: Illinois Research & Educational H.; Internalmedicine; Unmarried; Glenwood City, Wis.HOTCHKISS, JOHN ROBERTBorn Sept. 19, 1932; Wheaton College, A.B., 1955;Intern.: Billings; Internal medicine; Unmarried;A.O.A.; Route 1, Box 261 A, Candler, N.C.HYDE, JOHN ROBERTBorn Aug. 6, 1934; Pomona College, A.B., 1955, U.of Chicago, M.S., 1957; Intern.: Minneapolis GeneralH.; Internal medicine; Unmarried; 456 South Spald­ing Dr., Beverly Hills, Calif.KLEIN, MADELINEBorn Mar. 13, 1935; Mount Holyoke College, A.B.,1956; Intern.: Kings County H., Brooklyn; Specialtyundetermined; Unmarried; 22 Esplanade, MountVernon, N.Y.LAMBETH, JAMES THOMASBorn Feb. 13, 1936; Seattle U., S.B., 1957; Intern.:Fitzsimmons General H., Denver; Radiology; Mar­ried; One child; Route 1, Box 454, Vashon, Wash.LEONG, WILLIAM, JR.Born Nov. 20, 1935; Stanford U., A.B., 1956; In­tern.: Los Angeles County H.; Pediatrics; Unmarried;165 Madison St., San Francisco, Calif.MESSNER, RONALD PIERCEBorn July 15, 1935; Oberlin College, S.B., 1957;Intern.: University H., Cleveland;Medicine; Unmar­ried; 6134 N. Hamilton, Chicago 45.MONTO, GEORGE LUTHERBorn Mar. 2,1936; Illinois College, S.B., 1957; In­tern.: Blodgett Memorial H.; Tropical medicine;married; 1117 North Sixth St., Springfield, Ill.MOORE, HALLIE ELIZABETHBorn Jan. 4, 1936; Stanford U., A.B., 1957; Intern.:Cook County H.; Pediatrics; Unmarried; 720 S. Wol­cott, Chicago 10; 2010 Yolo Ave., Berkeley, Calif.NASH, HOWARD ALLENBorn Nov. 5, 1937; Tufts U., S.B., 1957; Intern.:delayed for research work at Billings; Physiology;Unmarried; 67 School St., Malverne, N.Y.NECHELES, THOMAS FABIANBorn Oct. 1, 1933; U. of Chicago, A.B., 1952, B.S.,1957, M.S., 1958, Ph.D. 1961; Intern.: New EnglandMedical Center, Boston; Internal medicine; Married;Three children; 5021 S. Woodlawn, Chicago 15.OLSEN, HOMER EDWARDBorn Mar. 23, 1935; Luther College, Decorah, Ia.,S.B., 1957; Intern.: U. of Oregon H.; Psychiatry ormedicine; Unmarried; Climax, Minn.OLSON, ROBERT PAULBorn Sept. 28, 1928; Cornell College, Mt. Vernon,Ia., A.B., 1950, U. of Illinois, M.S., 1952; Intern.:Presbyterian-St.Luke's H.; Internal medicine; Mar--=_...J. �� tIT ","T�_ .. L A • __ I'""L! .. n.MEDICAL ALUMNI BULLETIN 7CLASS OF 1961PACHMAN, LAUREN MERLEBorn Mar. 16, 1937; Wellesley College, A.B., 1957;Intern.: Philadelphia General H.; Immunology of pe­diatrics; Unmarried; 8133 S. Jeffery Ave., Chicago 17.PACKOVICH, MILAN JOHNBorn Jan. 13, 1936; Hiram College, Hiram, Ohio,A.B., 1957; Intern.: Robert Packer H., Sayre, Pa.;Internal medicine; Unmarried; 3426 Mahoning Ave.,N.W., Warren, Ohio.PAK, CHARLES YONG CHULBorn Nov. 27, 1935; U. of Chicago, S.B., 1958;Intern.: Billings; Internal medicine; Unmarried; 155Muliwai Ave., Wahiawa, Hawaii.PERLMAN, ROBERT LOUISBorn Aug. 15, 1938; U. of Chicago, A.B., 1957, B.S.,1958; Intern.: delayed for research work at Billings,biochemistry; Specialty undetermined; Unmarried;5448 S. East View Park Ave., Chicago 15.PREISMAN, ROBERT ARTHURBorn Sept. 13, 1936; Ohio State U.; Intern.: Cin­cinnati General H.; Specialty undetermined; Unmar­ried; 916 Highland Rd., Charleston, W. Va.READE, DERALD MAXBorn Oct. 3, 1929; U. of South Dakota, S.B., 1959;Intern.: Wesley H., Wichita, Kans.; General practice;Married; One child; Canton, S. Dak.RENNERT, OWEN MURRAYBorn Aug. 8,1938; U. of Chicago, A.B., S.B., 1957;Intern.: Billings; Biochemistry & pediatrics; Un­married; 195 Bennett Ave., New York, N.Y.ROSI, PETER SHAMBAUGH LOUISBorn Mar. 4, 1937; Swarthmore College, A.B., 1957;Intern.: Henry Ford H., Detroit, Mich.; Surgery;Married; One child; A.O.A.; 2499 W. Grand Blvd.,Detroit, Mich.; 10162 Longwood Dr., Chicago 43.SCHMID, PHILLIP GEORGEBorn Jan. 30, 1935; Carleton College, A.B., 1957;Intern.: Billings; Internal medicine; Unmarried;13206 Holdridge Rd., Silver Spring, Md.SHANNON, JOSEPH ROBERTBorn Nov. 14, 1930; Loyola U., S.B., 1952; Intern.:Muhlenberg H., Plainfield, N.J.; Internal medicine;Unmarried; 9553 S. Winchester, Chicago 11.SHAPIRO, BERTRAND JAYBorn Aug. 24, 1936; U. of California at Los Angeles,1957; Intern.: Indefinite; Internal medicine; Unmar­ried; A.O.A.; 35 Summerholme Pl., Hillsborough, Calif.SKOSEY, JOHN LYLEBorn Jan. 19, 1936; Southern Illinois U., A.B., 1957;Intern.: Billings; Internal medicine; Married; Route1, Clinton, Ill.SPINKA, RONALD BARRYBorn Feb. 16, 1936; U. of Illinois, S.B., 1958; In­tern.: Los Angeles County H.; Internal medicine orpsychiatry; Married; 1127 W. Farwell, Chicago 26.SPRATT, JAMES LEOBorn Jan. 27,1932; U. of Chicago, A.B., 1953, Ph.D.,1957; Intern.: U. of Iowa H.; Pharmacology; Mar­ried; 7804 S. Luna, Stickney Twp., Ill.STUCKER, THOMAS BERNARDBorn Feb. 27,1936; St. Joseph's College, A.B., 1958;Intern.: U.S. P.H.S. Hosp., Staten Island 4, N.Y.;Internal medicine; Married; 9558 S. Seeley Ave.,Chicago 43.8 MEDICAL ALUMNI BULLETINTHE SENIORSWANSON, WENDEL BROWMANBorn Oct. 5, 1930; Augustana College, Rock Island,Ill., A.B., 1952; U. of Chicago, M.S., 1958; Intern.:Butterworth H., Grand Rapids, Mich.; Radiology;Married; 8927 Harrison Ave., Rockford, Ill.TAGATZ, GEORGE ELMOBorn Sept. 30, 1935; St. Olaf College, Northfield,Intern.: Western Reserve Univ. H.; Internal medicine;Unmarried; 510 Geneva Ave., Bellwood, Ill.THOMASON, RICHARD DONALD, JR.Born July 31, 1930; Intern.: delayed for researchwork at Rigshospitalet, Copenhagen, Denmark; An­esthesiology; Married; One child; 9945 Harnew Rd.,Oak Lawn, Ill.TIETZ, CHARLES ALBINBorn Sept. 30, 1935; St. Olaf College, Northfield,Minn., B.A., 1957; Intern.: Minneapolis General H.;Internal medicine; Unmarried; 1711 George St., LaCrosse, Wis.TINNIN, LOUIS WATSONBorn Mar. 7, 1932; U. of Chicago; Intern.: GorgasH., Canal Zone; Psychiatry; Married; Three children;Box 526, Balboa Heights, Canal Zone.TOMPKINS, RICHARD LEOBorn Sept. 27, 1935; Walla Walla College, B.A.,1957; Intern.: Los Angeles County H.; Surgery; Un­married; 10628 N.E. Fargo St., Portland, Oreg.TRACY, RICHARD EVERETTBorn Apr. 30, 1934; U. of Chicago, A.B., 1955,Ph.D., 1961; Intern.: Presbyterian H.; Denver, Colo.;Pathology; Unmarried; 2525 Applegate Ave., KlamathFalls, Oreg.URSCHEL, CHARLES WILLIAMSBorn Jan. 26, 1936; Carleton College, A.B., 1957;Intern.: Columbia-Presbyterian H., N.V.C.; Internalmedicine; Married; One child; A.O.A.; Box 415;Mentone, Ind.THE GROOMING OF A CHAMPIONA month or so ago, as you well rea­lize, the newspapers were full of com­ments and reports about the heavyweight championship fight. While read­ing these accounts I kept thinking aboutthe concept of what it takes to makea champion. Most people would answer,"Blood, sweat, and tears," but I couldn'trefrain from thinking it was somethingmore basic. Any champion would, al­most without exception, attribute hissuccess to the experience gained in theearly four-round fights that enabled himto cope with all kinds of presentingsituations. I recently interviewed sev­enty-seven prospective candidates, who are striving to be champions in theirown right. In preparation for the mainevents, these individuals had similarfour-round bouts against the same op­ponents.It was the fall of 1957 in Chicago;the group had just arrived from theirrespective training camps where theyhad been preparing for three or fouryears and were now ready for the offi­cial weigh-in and pre-fight instructions.A bit nervous, the seventy-sevencandidates had gathered at Abbott'sGym and exchanged mutual greetingsand talked of previous encounters, de­feats, and victories, and of the new chal- lenge. I'll never forget how we allthought one of the group, a characterin a beard and a leather jacket, hadwandered into the Gym by mistake.This, of course, was "Matthew, Mark,Luke (Sigmund)" Dumont, soon to for­sake his Second City attire for an ana­lyst's couch.By unanimous consent both sides hadchosen for referee a person whose rep­utation for fairness and neutrality longpreceded him; he was nicknamed,"Zwitterion," but better known as"Uncle Joe."A great hush fell over the group asZwitterion strode into the room. AfterMEDICAL ALUMNI BULLETIN 9CLASS OF 1961WALGREN, KENNETHBorn Feb. 28, 1930; Beloit College, A.B., 1952, U. ofWisconsin, M.S., 1957; Intern.: U. of Iowa H.; Medi­cine; Married; Pearl City, Ill.WENTZ, DENNIS KEITHBorn Sept. 1, 1935; North Central College, Naper­ville, Ill., A.B., 1957; Intern.: U. of Maryland H.; In­ternal medicine; Unmarried; Napoleon, N. Dak.WILLIAMS, CHARLES EVERETTBorn Sept. 4, 1935; Yale U., B.A., 1957; Intern.:Highland-Alameda County H., Oakland; Internalmedicine; Married; Two children; 10359 Monte MarDr., Los Angeles, Calif.WILSON, THOMAS WILLIAMBorn Oct. 5, 1936; Southern Methodist U., S.B.,1957; Intern.: Vanderbilt U. Hosp.; Pediatrics; Mar­ried; 526 Lamont Ave., San Antonio, Texas.WINEMILLER, ROBERT HAROLDBorn May 27,1935; DePauw U., A.B., 1956; In­tern.: U. of Michigan H.; Internal medicine; Mar­ried; One child; 412 W. Grantley, Elmhurst, Ill.WOODBURY, LINDA PLZAKBorn Apr. 30, 1937; U. of Chicago, A.B., S.B., 1957,S.M., 1959; Intern.: Passavant Memorial H., Chicago;Obstetrics & Gynecology; Married; 5715 Drexel Ave.,Chicago 37, Ill.; 560 N. Washington, Hinsdale, Ill.WOODWARD, ROBERT WARRENBorn Mar. 4, 1936; Earlham College, A.B., 1957;Intern.: Highland-Alameda County H., Oakland,Calif.; Obstetrics & Gynecology; Unmarried; 831 Bay­ard Pl., San Diego 9, Calif.WRIGHT, GEORGE DONALDBorn Mar. 27, 1932; Eastern Illinois College, A.B.,1957; Intern.: U.S. Army, Ft. Benning, Ga.; Pediat­rics; Married; two children; 205 1st St., Charleston,Ill.a short welcome he instructed the groupabout the rules and assured all that hewould do everything in his power tomake sure the contest was a fair one.He then introduced some of the oppo­sition, who told us that as seasonedveterans they would help each gladiatorin his quest for recognition; with thisreassurance everyone felt more at home.At a parting shot, Zwitterion told thecontenders about the scoring system ofbouts held at Brown's Billings Garden.He said the ringside judges would eval­uate each fighter's performance, grad­ing each individual on his own ability,in order to eliminate any internecinecompetition. These evaluations wouldbe kept in strict confidence by thejudges and the referee unless the fighterWas KO'd. Within a short time each challengerhad his equipment ready and donnedhis new white robe and stepped into thering to meet the first opponent, "K. O.Cadaver." The opening seconds of thecontest had everybody backpedallingand protecting his nose as there wassomething "fishy" about K.O.'s style.But with some great corner advice fromthat old pro, Coach Kabe, dissection ofK. O. 's style was not difficult and hewas literally torn limb from limb.Midway through the first round abig serendipity punch almost threweveryone, even the compulsive fighters,off guard, but with the multiple-choicedefense of the opposition, all gladiators"Forbes"ed ahead. The closing secondsof the first round were used to get asecond wind. Some, however, managed to take time to look at the famous whiteand gray columns that support thespectacular circle of Willis, while othersgot a glimpse of the celebrities in theaudience including the dashing SylvianFissure and her pineal body.At the end of the first round, Zwit­terion and the promotional judges com­pared notes and to the amazement of allthe young fighters, the first round wasawarded to them. By this time a muchneeded three-minute rest was in orderso each fighter retired to his own cor­ner. In rehashing the first round withtheir respective managers and promot­ers, many thought the opposition hadthrown some border line punches. Forexample, the "shrinking" of womengreat with child and the "tadpole, small­fog" punch. All in all, however, it was a10 MEDICAL AL UMNI BULLETINprofitable round and a good one to haveout of the way.In the second round, the end-organsresponded to the stress put on them,even though the opposition was almostpathological in its sliding and grossdemonstration of near post-mortemtactics. "Rabbit" punches and unsterileconditions went undetected by the ref­eree and it was the �oncensus of ring­siders that the Home for the Incurableswas the next stop for the young neo­phytes. By the middle of the secondround the boxers were "worming" theirway out of anaplastic situations. Latein the second round the long awaitedopening finally came-some boxers"elected" to vary their offense but mostof them concentrated on a new typeconsisting of probing and feeling forweakness in the physical make-up ofthe opposition. Fingers were prickedand a little blood was spilled in thebattle. During one scuffle in the cornerof the ring a near disaster occurred assome uncouth had left a formed, solid,ring-stool, not a sign of culture, and itdrew 4-plus sensitivity from the crowd.Spirits were running high as the oppo­sition varied its offense in vain attemptsto stump the now somewhat confidentyoung gladiators. Speaking of spirits,the opposition tried to soft-soap theboys about the pharmaco-sociologicalevils of ethanol, i.e., "one drink is toomany, and a thousand is not enough.""I couldn't tell that pretty blonde at theAdmitting Office that 1 have trouble with mynature, Doc, so 1 told her 1 had a sore throat."Everybody turned to look at "IrishJoe" and "Lacnnec" Goldstone. Thiswas an exhausting round and the be­tween-round rest seemed even shorterthis time. The third round opened fiercely­some of the fighters "surged," some"shrunk," some back-"pedseled," andsome "med" the opposition head-on.The group was now relying strictly oninstinct with corner advice from theirable handlers: Cecil, Loerb, J. Garrett,Nelson, and that old veteran, MerckSmerek. Under their encouraging guide,the boxers gained new stature and si­multaneously the opposition broughtforth its best offense: Terrible TedPullman tried to confuse the youngneophytes with constant harrassing,Dandau Landau caused much conster­nation with his changing mood, and abig tall "pseudopod orthopod" and hisshort "Napoleon" partner, the "apple"of his eye, gave some of the fighters agood working over, cutting them uppretty badly. In addition each fighter hadto survive a medical regimen consistingof 30 mg Palmer and 7.5 mg Kirsnerdaily.While on exhibition tour to Mantenosome shocked the opposition with thei�great display of diversified talents, name­ly guitar-strumming and folk-singing, sothat the squirrel-keepers were unable tosleep and subsequently reported them.When the bell rang one fighter hadbeen KO'd and two had gone down forthe nine count only to rise again-therest came through bloody but unbowed.Between the third and fourth roundsmost fighters took time out to look atthe future opposition that would meanmore prestige and opportunity to ex­press individual talents. The fourthand final round was to be a real pleas­ure, the fighters had the battle essen­tially won and this round was to sharp­en up their techniques. Each move thefighter made was planned and had itsown meaning-blending together to givea confluent approach to each presenting situation. Instructions from corner-menhad even greater meaning; skin .lesionsand projected silhouettes were no long­er a mystery. Ming Fu and traveloguesdown the "afternoon Path" weren't toobad and even the Ovary Tower wastolerable. Most of the gladiators seemedto fare pretty well during this round:one ruptured a heterophile, one sud­denly acquired a "pica" for lead pipes,and another small group picked up astrange bug causing that fatal disease,"altar fever," while still others stayedat home and caught up on their math(multiplication). At the end of thefourth round the referee held theirhands in victory and assured them theyall had made a good showing.So, as you can see, without the helpof the four-round bouts these youngfighters could not have graduated to thebigger challenges ahead. Without thehelp of all their sparring mates andmany more, the final result could nothave been achieved. In spite of theirway of presenting obstacles necessaryfor grooming a champion, the entireopposition will always be rememberedgratefully by the gladiators-for with­out them advancement would have beenimpossible and championship out of thequestion!CHARLES TIETZMEDICAL ALUMNI BULLETIN 11SENIOR SESSIONSCIENTIFICThe Fifteenth Annual Senior ScientificSession was held on the afternoon and eve­ning of Tuesday, June 6. Between sessionsthe presenting seniors and the faculty haddinner together in the Doctors DiningRoom. The committee in charge was LeonO. Jacobson, '37, Chairman, John D. Ar­nold, '46, Frank Fitch, '53, Murray Rab­inowitz, and Lloyd Roth, '52.Biological Effects of thePolyoma Virus inEmbryonal andNewborn RatsBy DAVID G. ANDERSONPathologyThe polyoma virus, originally iso­lated from mice with spontaneouslymphatic leukemia, can produce mul­tifocal tumors of diverse histogenesisin various species. Although the rat isknown to be susceptible to oncogenesisby the polyoma virus, the tumor re­sponse in this species is reportedly sin­gular, i.e., only renal sarcomas resultfollowing neonatal injection of the virus.Our studies on the effects of the poly­oma virus were initiated by an inci­dental observation. Newborn rats wereinjected by various routes with leu­kemic cell suspensions of AKR mice inan attempt to transplant this leukemiautilizing the phenomenon of acquiredimmunological tolerance. The recipientrats developed leukemia or multicentricmalignant tumors or both. Histologi­cally, the multifocal neoplasms wereidentical with those known to be causedby the polyoma virus. Experimentswere then designed to identify and seri­ally propagate the causative agent.Hemagglutination and hemagglutination­inhibition tests indicated that the causeof these multiple mesenchymal andepithelial tumors was the polyoma vi­rus. This virus, obtained from homog­enates of induced rat tumors, was thencultured in vitro using monolayers ofmouse embryonic kidney cells. The on­cogenic range of three lines of polyomaviruses was compared: a) virus con­tained in leukemic AKR suspensions;b) virus cultures obtained from Dr. J.Hartley, N. I. H.; and c) virus culturesgrown in our laboratory. It was ob­served repeatedly that the tumor re­sponse to leukemic AKR cell suspen­sions was characterized by a multiplicityof primitive neoplasms not seen in re- sponse to cultured virus preparations.The range of induced tumors was con­siderably enhanced following rapid, se­rial in vivo passage of the polyomavirus. Furthermore, the oncogenic ef­fects of the three studied virus lineswere dependent upon the routes of inoc­ulation and the age of the recipients.Adult animals were not susceptible totumorigenesis by the polyoma virus.Most tumors developed in rats less thanthree days of age were benign. Undif­ferentiated mesenchymal tumors wereseen in neonatal rats injected intra­venously or those injected in utero.Thus, the degree of histological differ­entiation of the induced tumors was de­termined by host factors rather thanfactors of the causative agent.Family Care After a ThousandYears-A Crisis in the Tra­dition of St. DymphnaBy MATTHEW P. DuMONTPsychiatryThe author spent an academic quar­ter at the world's oldest family-carecolony for mental patients in Geel,Belgium.The colony's legendary origins re­volve about the Irish princess, Dymph­na, who was martyred in 700 A.D. byher possessed father as she attemptedto escape his incestuous advances. Pil­grimages of mentally ill patients weremade to her shrine and as the pilgrimsbegan to appear within the homes of thetownspeople, a tradition of family caretook root and has persisted to the pres­ent.The colony which has twenty fourhundred chronically ill, psychotic, andmentally deficient patients is now super­vised by a staff of psychiatrists. Its continuance is currently jeopardized,however, by sources in the Ministry ofHealth unsympathetic with other thancustodial forms of mental care.The forty-seven families who wereonce in the program but are no longeraccepting patients were interviewedand compared with an equal number offamilies who are continuing to acceptpatients. Factors encouraging familiesto accept patients included a traditionof patient care, rural location, farm orwhite-collar work of modest income,family solidarity, and tractable patients.Eighty per cent of the families nowcaring for patients would accept othersif the current patients were to die orleave. Despite a steadily rising standardof living, the inroads of an industrialcivilization and static levels of remu­neration, the tradition is not dying outand would persist if allowed to.Effects of Medium Fatty AcidConcentration, Epinephrine, andGlucose on l-C't-Palmirare Ox­idation and Incorporation intoNeutral Lipids by SkeletalMuscle in vitro.By R. PHILIP EATONMedicineThe rate of oxidation of palmitate-1-C14 to C1402 by rat skeletal musclein vitro was shown to increase marked­ly as a function of the medium concen­trations of free fatty acids (FFA).Neither epinephrine nor glucose hadany important effect. The rate of in­corporation of palmitate-t-Cl+ into tis­sue-neutral lipids was also increased byhigher levels of medium FF A. At anygiven concentration of medium FF A,this incorporation was also increased byglucose but was not altered by epine­phrine. It is concluded that the deposi­tion of triglyceride that has been re­ported to occur in muscle after the ad­ministration of epinephrine or norepine­phrine is due to the effects of these hor­mones on serum FF A levels and not toa direct effect on muscle metabolism.The profound effect of medium FF Aconcentration on the rate of FF A oxi­dation suggests that regulation of theserum concentrations of this labile lipidfraction may be of central importancein determining overall rates of bodymetabolism.12 MEDICAL ALUMNI BULLETINA Study of the Kineticsof ErythropoiesisBy EDWARD V. FILMANOWICZ, JR.Medicine (ACRH)Some aspects of the kinetics oferythropoiesis have been studied in or­der to examine the action of the eryth­ropoietic-stimulating factor, erythropoie­tin. The transfusion-induced polycythe­mic mouse was chosen because endo­genous erythropoiesis is eliminatedwithout harmful effects to the animal.A purified preparation of erythropoie­tin, a glycoprotein obtained from plas­ma of sheep rendered anemic by admin­istration of phenylhydrazine, was usedto stimulate erythropoiesis.The amounts of erythropoietin re­quired for minimal and maximal re­sponse, as measured by reticulocytosis,were established. Erythropoietin poten­cy was expressed in units of activity asdefined by Goldwasser and White. Sixunits of erythropoietin, in a single sub­cutaneous injection, produced a maxi­mal reticulocytosis of 1.3 per cent onthe third day. Decreasing responseswere obtained with decreasing amounts;as little as 0.75 unit was followed by0.26 per cent reticulocytes. The timeand duration of peak response were in­dependent of the dose.Following a single maximum stimu­lating dose, erythrocyte precursorswere counted in splenic red pulp ofgroups of animals sacrificed daily. Pro­erythroblasts increased from 0.2 percent of nucleated cells to 0.9 per centin 24 hours. Normoblasts increasedfrom 0.0 to 9.6 per cent in 72 hours.Also of interest was the observationthat more erythorpoiesis occurred sub­capsularly than centrally.In order to examine the effect oferythropoietin on proerythroblasts andnormoblasts, a second dose was admin­istered to one group of mice at 24 hourswhen only proerythroblasts were pres­ent, and to another group at 48 hourswhen normoblasts were in abundanceand increasing rapidly. In neither in­stance did reticulocytes appear earlierthan they did following a single dose,nor was the magnitude of reticulocy­tosis at 72 hours enhanced. From thesedata it is concluded that erythropoietinacts primarily by stimulating differen­tiation of primitive reticulum, and noeffect on differentiated erythrocyte pre­cursors (proerythroblasts or normo­blasts) could be detected. Production of Erythropoietin bythe Isolated Perfused KidneyBy ELIZABETH FORDMedicine (ACRH)Recent basic studies and clinical in­vestigations have supported the thesisof Jacobson, et at. that the kidney is amajor source of erythropoietin, a hu­moral factor which stimulates erythro­cyte production. Various approaches tothis problem have been reported in theliterature. These include nephrectomy,ureteral ligation, irradiation, etc. Themost direct approach has been perfu­sion of the isolated kidney under vari­ous conditions with bio-assay of theperfusate for erythropoietic activity.Reports of such investigations have re­cently appeared in the European andAmerican literature. However, the pres­ent investigator questioned certain as­pects of the perfusion technique andbio-assay method employed. Therefore,the purpose of this investigation was tostudy erythropoietin in the perfusedkidney, using a simple in vitro perfu­sion apparatus and well standardizedbio-assay technique.In an attempt to stimulate erythro­poietin production and release, rabbitkidneys were prefused with heparinized,hypoxic, whole rabbit blood for threehours. Control data were obtained byemploying the same method, but usingwell oxygenated blood. A problem arosebecause the perfusion of blood throughthe isolated kidney resulted in the, re­lease of significant amounts of hemo­lysins as demonstrated by in »itrohemolysis of rat cells by the perfusateplasma. Extraction of the plasma bythe methods developed by Dukes andGoldwasser appeared to eliminate this.The extracted plasma could then be as­sayed by Fe59-uptake in the starved rator reticulocyte count in the polycythe­mic mouse. Results of the present in­vestigation suggest that under hypoxicconditions, the isolated, perfused, rabbitkidney produces a heat-stable erythro­poietic factor. This finding lends addi­tional support to the hypothesis thaterythropoietin is produced by the kidney.ney.Mechanism of RhodaneseActionBy JOHN R. GREENBiochemistryThe sulfur transferase, rhodanese,catalyzes the reaction: SSO=8 + CN- = SCN- + SO=3. All of these ions,which may be distinguished polaro­graphically using a dropping Hg elec­trode, give characteristic anodic dif­fusion currents. In contrast, crystallinebeef-liver rhodanese is characterized bya single, well defined cathodic current.It has thus been possible to determinequalitatively and quantitatively all com­ponents of reaction mixtures containingequivalent concentrations of rhodaneseand substrates or products. Reactionmixtures contained initially 0.5 mg ofcrystalline rhodanese and 10-100 m,u.moles of the appropriate ions in 0.1 mlof phosphate-borate buffer, pH 8.7, at250• Rhodanese treated with CN -pro­duced 2 equivalents of SCN- per moleof crystalline enzyme with equivalentdisappearance of CN -. Similarly rho­danese treated with SO=3 produced 2equivalents of SSO=3 per mole of crys­talline enzyme with equivalent disap­pearance of SO=3. Disappearance ofthe cathodic current of the active en­zyme occurred in both of these reac­tions and also when the enzyme wasallowed to denature at 250• In contrast,when rhodanese was treated withSSO=3' no change occurred in eitherthe cathodic current of the enzyme orthe anodic current of SSO=3. The datademonstrate that crystalline beef-liverrhodanese is an enzyme-substrate inter­mediate containing two activated sub­strate sulfur atoms per molecule. Theseresults are consistent with a double dis­placement mechanism of enzyme cata­lysis.In vitro Effect of Insulin andThyroxine on Protein andNucleic Acid Synthesisof Rabbit BoneMarrowBy THOMAS F. NECHELESPhysiology (ACRH)Bone-marrow function in the intactanimal is known to be under hormonalcontrol. Erythropoiesis, for example, isregulated at least to some extent by thehumoral factor, erythropoietin. How­ever, the role of other general anabolichormones in this tissue has been ill de­fined. The present study reports somein vitro effects of two major anabolichormones, insulin and thyroxine, onprotein and nucleic acid synthesis andturnover in bone marrow.Fasting young rabbits were sacrificed,myeloid marrow rapidly removed fromboth femurs, sectioned, and incubatedMEDICAL ALUMNI BULLETINin Krebs-bicarbonate buffer with appro­priate C14-labeled precursors. Afterincubation for one hour under 95 percent O2, 5 per cent CO2, the protein ornucleic acid adenine was isolated andpurified. Insulin (0.01 unit/rnl) gave25.9 ± 1.2 (mean ± SE) per cent stim­ulation in the incorporation of histi­dine-X-C14 into peptides. Alkali-inacti­vated insulin was without significanteffect, as was addition of glucose oramino acids. Thyroxine (10-7 M)elicited a 49.4 ± 2.1 per cent increasein amino acid incorporation. Triiodo­thyronine acted similarly, but diiodo­thyronine was without significant effect.Although it appeared that the additionof glucose promoted the stimulatoryeffect of thyroxine, incorporation ofgluccse-U'-Cw into peptides was de­pressed in the presence of this hor­mone; Response to thyroxine had asharp optimum at 10-7 M with de­pression of the rate of amino acid in­corporation at high concentrations.Insulin also apparently promoted anincrease in net synthesis of both RNAand DNA whereas thyroxine appearedto stimulate primarily DNA synthesis.In addition, incorporation of glycine-2-C14, but not adenosin-s-Ct! into RNAand DNA was markedly stimulated bythyroxine.It is concluded that insulin and thy­roxine can increase incorporation ofamino acids into peptides and of cer­tain precursors into nucleic acids ofbone marrow in vitro thus providingevidence for the hypothesis that thesehormones act directly on bone marrowin the intact animal.The Challenge ofHypersensitivityBy LAUREN PACHMANMedicineAlthough the tubercle bacillus and itsrelation to antibody formation has re­ceived much attention, many aspects ofthe immunological mechanism are un­known. It is well established, however,that sensitivity to a tuberculin antigen,PPD, can be transferred by leukocytes,but not by whole circulating antibody.In the present study, two methods oflabelling were used in an attempt toclarify the mechanism of interaction ofthe tubercle bacillus with both sensi­tized cells and known hyperimmunesera. Immune sera were produced inguinea pigs by serial injections of livingRIRv bacilli. The sera obtained fromthese animals was inactivated, pooled, and adsorbed on normal sheep red cells.Titration was done, to demonstrate thepresence of antibody, using sheep cellswhich had been tanned and coated withPPD-RT, according to the method ofBoyden and Sorkin. The whole serumwas then conjugated with fluorosceinthioisocyanate (Coons' technique) andagain titred with the tanned PPD-RTcoated cells in an effort to detect micro­scopically the antigen-antibody reac­tion on the red-cell surface. This couldnot be done however because the fluoro­scein lysed the red cells. In the nextexperiment, the serum was incubatedwith whole tubercle bacilli which hadbeen effixed to slides in various manners-with heat, methanol, acetone, orformalin. Inactivated immune and nor­mal labelled serum, with and withoutthe addition of complement, was usedand in no case was the specific adher­ence of the labelled antibody demon­strated. The same negative results wereobtained when the live bacilli wereground on glass beads and then incu­bated with the various sera. In addi­tion, when the immune serum was frac­tionated and the gamma and beta com­ponents labelled with the fluorescentdye, no adherence was found.The next phase of work will involve1) defatting the bacilli and 2) usingultrasonic vibration to disrupt the bacilliin a final effort to apply the Coon's tech­nique to the tubercle bacillus and itscirculating antibody.In the second series of experiments,the PPD-RT was labelled with p3l andallowed to react with spleen-cell suspen­sions from normal and recently sensi­tized guinea pigs (10 days). The cellswere washed and counted. It was foundthat the normal cells took up as muchof the labelled antigen as did the sensi­tized cells, but that the level of uptakedoubled when either the immune or normal cells were suspended in activesera from the donor animal. This didnot occur when the sera were inacti­vated. Further work on this is now inprogress.Study of Molecular Specificityby Use of Spread Protein Mono­films at Air-Water InterfaceBy CHARLES Y. C. PAKMedicineBeef, pork, and sheep insulins areknown to differ structurally in amino­acid composition. Previous studies ofinsulin cross-reactions have suggestedimmunologic identity of insulins fromdifferent species. But it is doubtfulwhether any of the usual immunologictechniques permits precise quantitativedetermination of differences in reactionof insulin with antibody.Such quantitation, however, is madepossible by using a new techniqueadapted to measure specific molecularinteraction between the insulin mono­layer and serum proteins in the subso­lution at the air-water interface.In Tris buffer, the difference in re­action between pork and beef insulinmonofilms was 2 S per cent when thesefilms were reacted with normal bovinegamma globulin, while it was 23 percent between beef and sheep insulinmonofilms with normal human gammaglobulin. The gamma globulin of a dia­betic patient receiving insulin reacted3S per cent more with beef- than withpork-insulin monofilm,Species variation could not be dem­onstrated in McIlvaine's buffer, usingother serum proteins, or with aged solu­tion of gamma globulin.It is presumed that the three speciesof insulin monofilms differ in theirmolecular structure or reactive sites,and that these sites are exposed by un­folding of the molecule at the interface.The behavior of the insulin monofilmstherefore reveals molecular character­istics of the protein which may not beapparent by other methods.Urinary Electrolyte ExcretionDuring and After Cardio­pulmonary By-PassSurgeryBy DERALD MAX READESurgeryAlthough the literature on the rela­tionship of surgery and serum electro-14 MEDICAL ALUMNI BULLETINlytes is voluminous, surprisingly fewstudies have been done on urinary elec­trolytes· during surgery and the imme­diate post-operative period. The workof others does seem to agree that thereis a post-operative decrease in urinaryexcretion of sodium lasting about fivedays, but the range of values and eventhe average values are not in closeagreement. The cases studied have beenfrom the field of general surgery, thor­acic and cardiac surgery, but not open­heart surgery with cardiopulmonary by­pass. It has been an impression that pa­tients placed on extra-corporeal circula­tion tend to lose more urinary sodiumduring the immediate post-operative pe­riod than do the usual surgical cases.Twenty patients undergoing cardio­pulmonary by-pass for the correctionof various congenital cardiac defectswere studied pre-operatively, duringsurgery, and post-operatively for fivedays. Studies included serum and twen­ty-four hour urinary electrolytes.Marked increases in sodium, potassium,and chloride excretion were observedin the early post-operative period. Con­sequently results of the study supportthe clinical impression that electrolyteexcretion following cardio-pulmonary by­pass differs from that observed afterother surgical procedures.A Study of the Action and Ef­fects of 5', 5', 5' - Trill uoroleucineon a Mutant of E. ColiBy OWEN M. RENNERTBiochemistryA number of amino acid analogues,such as the fluorophenylalanines, thieny­lalanine, azatryptophane, azaserine, nor­leucine, methallylglycine and selenome­thionine have been shown to inhibit thesynthesis of proteins by microorgan­isms. With the exception of selenometh­ionine, these analogues exert their effectwithout themselves being incorporatedto any significant extent into proteinsproper. Selenomethionine, on the otherhand, can completely replace methio­nine in the proteins but does not sub­stitute for the cysteine requirement ofthe organism.In the experiments reported here, itcould be demonstrated that 5', 5', 5'­trifluoroleucine can substitute in part,in the presence of leucine in proteins,for a leucine-requiring mutant of E.coli and that this organism can beadapted to grow exclusively on trifluoro­leucine. Effects of Anterior PituitaryHormones on Metabolismin Adipose TissueBy JOHN L. SKOSEYPhysiologyAdipose tissue has been shown to behighly active metabolically, and muchinterest has been generated in the hor­monal control of this metabolism. Theadrenocorticotropic hormone is of spe­cial interest, having been shown to be apotent factor in causing the release offatty acids from adipose tissue andotherwise altering adipose-tissue metab­olism, independent of the effects it hasupon the adrenal cortex.The effect of anterior pituitary hor­mones on the utilization of acetate bypaired testicular fat pads of the rat in­cubated in vitro was studied by meas­uring the incorporation of radio-acetateinto protein and fatty acid. It was ob­served that adrenocorticotropic, growth,thyroid-stimulating, and interstitial­cell stimulating hormones all inhibitedacetate utilization. The activity of thelatter three hormones was attributedto contamination of the preparationswith adrenocorticotropic hormone.It is not certain whether the effectobserved is another "extra-adrenal ef­fect" of adrenocorticotropic hormone,or if in this instance, metabolism in adi­pose tissue is being altered in a mannersimilar to that in which metabolism inthe adrenal cortex is altered by thishormone.Comparative Radiosensitivity ofthe Gastrointestinal TractBy RICHARD THOMASONPathologyThe increased use of high-voltage ir­radiation in the treatment of cancer hasresulted in a need for further evalua­tion of possible side effects. Although it is generally acknowledged that thegastrointestinal tract is relatively radio­sensitive there have been no studies toindicate whether one portion of thetract is more sensitive than another.This study was undertaken to contributeinformation on this subject.Adult, albino Sprague-Dawley ratswere anesthetized and a loop of intes­tine, either stomach, duodenum, jeju­num, ileum or colon, was exteriorized.The rest of the animal was shieldedfrom irradiation and the individual loopwas given 2000, 3000 or 4000 r from aMaxitron X-ray machine at 250 KVand 30 rna using a I-mm A! and l-mmCu filter resulting in a dose rate of 70 rper minute. Animals were examinedgrossly and microscopically. Compari­sons were made of the pathologicalfinding at varying doses, intervals andsegments irradiated.Grossly at the end of twenty days,obstruction of the jejunal, ileal, andcolonic segments irradiated was promi­nent at the site of irradiation with se­vere dilatation proximal to this site.This was not observed when either thestomach or duodenum was irradiated,although in all segments peritoneal ad­hesions were prominent at the site ofirradiation. Microscopically, the I cellulardamage to all segments appeared com­parable at comparable doses but notnecessarily at comparable intervals.Mucosal necrosis, submucosal edema,telangiectasia and muscularis necrosiswas seen in all segments at the highestdose of 4000 r. With lower doses, mus­cularis necrosis was not a common find­ing. Fibrosis and scarring at the site ofirradiation were common in animalsthat survived for more than one month.This study indicates that all portionsof the gastrointestinal tract are equal­ly radiosensitive, but that death of theanimal is more commonly observedwhen the small intestine is irradiatedbecause obstruction is more easily pro­duced due to the small lumen.Cognitive Activity WithoutAwarenessBy LOUIS W. TINNINPsychiatryA simple extension of previous ex­periments using hypnosis demonstratesthat cognitive activity can utilize cuesand can proceed without the subject'sawareness.Three university student volunteerswere hypnotized to a deep amnesicMEDICAL ALUMNI BULLETIN 15trance by the technique of progressiverelaxation and were instructed in 'theuse of a cue system which would pro­vide the information necessary for solv­ing complex problems in the post-hyp­notic waking state. They were thenawakened and tested for recall. Whenamnesia for the trance was confirmedthe subjects were exposed to the cuesystem and the problem was presented:The subjects found that correct answersto the problems emerged spontaneous­ly into consciousness without their beingaware of the cognitive activity involvedin the solution.Several kinds of problems were usedwith varying levels of complexity. Onekind required the solving of mixed alge­braic expressions of the first andsecond degrees with two unknowns, theunknown values being presented bycues. Various cue systems were used,some obvious, some disguised. The sim­plest consisted of a card containing twocolumn of digits, shown to the subjectin the post-hypnotic state. In thetrance the subjects were told that onawakening they would unconsciouslysolve a problem, for which they wouldchoose the proper values of the un­knowns from the cue card. In somecases they were told what particularproblem would be posed; in others theywere given only the cue system to beused. Each time they were told that thecorrect answer would come to theirconscious minds when the solution wasasked for. The subjects found that cor­rect answers to the problems emergedspontaneously into consciousness with­out their being aware of the cognitiveactivity involved in the solution: eitherin the selection of the right digits touse as values for the unknowns, or inthe calculations involved in the solu­tion.BULLETINof the Alumni AssociationThe University of ChicagoSCHOOL OF MEDICINE950 East Fifty-ninth Street, Chicago 37, IllinoisSPRING 1961VOL. 17 NO.3EDITORIAL BOARDJESSIE BURNS MACLEAN, SecretaryWRIGHT ADAMS ROBERT J. HASTEJlLIItJOHN D. ARNOLD ELEANOR M. HUMPHREYSL. T. COCGESHALL HUBERTA LIVINGSTONERICHARD EVANS PETER V. MOULDERWALTER L. PALMER Other problems involved the deci-�.phering of codes, the extension of arith­metic; progressions, and various deduc­tions and ordering of material, all usingguides supplied under hypnosis."In all cases the subjects solved theproblems in the post-hypnotic stateand denied awareness of the mentalactivity involved. The results indicatethat complex cognitive activity can runparallel to, without intruding upon, con­scious activity.c Ilw/ftrll! !The Mechanism of NephroticHyperlipemiaBy RICHARD TRACYPathologyIn the condition of nephrotoxic ne­phrosis in rats, if dietary control iskept rigid by stomach-tube feeding of astandard diet, serum-lipid concentrationis directly related to 1) total 12-hoururinary protein output, 2) alpha-l glob­ulin concentration in serum, 3) alpha-2globulin concentration in serum; and itis inversely related to serum concentra­tion of 1) albumin, 2) beta-globulin,and 3) gamma globulin. These experi­ments have indicated that subcutaneousadministration of protein taken fromthe urine of nephrotic rats consistentlylowers serum concentration of totallipid in nephrotic rats if the concentra­tion of lipid is high at the onset, butnot if it is low. Injection of urine pro­tein also produces a diminution of totalurinary protein output, affecting the al­bumin excretion to a greater extentthan it affects the globulins and produc­ing a rise of serum alpha-2 globulins. Ofthese changes following urine injec­tion, the lipemia is consistently cor­related only with the urinary albumin­excretion rate. Serum-albumin concen­tration-changes are always found to be in the opposite direction from those tobe expected if hypoalbuminemia causeslipemia. These effects can be reproducedby injection of egg albumin and bygrowth of a transplantable tumor inthe nephrotic rat. It is concluded that,of all the urine and serum-protein elec­trophoretic factions that were measuredin this study, the daily urinary loss ofalbumin appears to be the most closelyrelated to the cause of the hyperlipemia.Electron Microscopy of N ephro­toxic Nephritis in the RatBy ROBERT H. WINEMILLERPathologyFor years investigators have inducedkidney disease in animals in their at­tempts to understand human nephritis.The most widely used technique in­volves anti-kidney serum. Much con­troversy exists over the findings.The nephrotoxic serum used herewas a relatively pure anti-basementmembrane-serum instead of the usualserum made against whole kidney. Theserum, produced in rabbits, was ad­sorbed against rat red-cells and heatedto 56° C. before use. Controls were in­jected with normal serum. Tissues werefixed in buffered osmic acid, imbeddedin plastic, sectioned, and examined withan electron microscope.The normal and control glomerularcapillary wall consists of 1) a singlelayer of endothelium, a very thin fenes­trated coat along the basement mem­brane, 2) the basement membrane, athin homogeneous strip, and 3) the epi­thelium, with narrow cytoplasmic "footprocesses," separated by slits, abuttingon the basement membrane.Four hours after injection, areas ofbasement membrane were denuded. Theendothelium was thickened and retract­ed, and in its place leukocytes ad­hered to the basement membrane.After eight hours, the basement mem­brane was focally thickened, due to ac­cumulation of basement membrane-likestrands and amorphous material. Theepithelium had no discrete foot proc­esses, but was a continuous cytoplasmiclayer. Intracapillary platelet-fibrinthrombi were seen. By one to nine daysa remarkable nephritis with alterationsin all layers of the capillary wall existed.This state is judged to resemble that ofhuman post-streptococcal glomerulo­nephritis.16A.O.A. ELECTIONSFour member of this year's' graduat­ing class were elected to Alpha OmegaAlpha when they were juniors. Theyare:Ronald P. MessnerPeter S. RosiBertrand J. ShapiroCharles W. Urschel.On May 15 seven seniors and threejuniors were initiated. The seniors are:'Arthur AuerbachFred CoeIvan DiamondPhilip EatonJerry FeinFloyd FriedJohn Hotchkissand the juniors:Joseph BaronRobert StagmanThomas Stockert.WiIIiam R. Barclay was electedfrom the faculty.Dean H. Stanley Bennett gave theA.O.A. lecture on "The Importance ofMembrane Movements in Cell Physiol­ogy."Archie Lieberman took the portraitsof Chancellor Beadle and Dr. Adams,and Charles Pak did the class cartoons.MEDICAL ALUMNI BULLETINDEATHS'91. William'M. Yockey, Decatur, Ill.,January 7, age 92.'97. W. Albert Cook, Tulsa, Okla., De­cember 13, age 85.'98. Fred C. Franke, Forest Park, Ill.,April 22, 1959, age 85.Hiram B. Young, Gurnee, Ill., July 6,age 86. ''99. William N. Keller, Steilacoom,Wash., January 14, age 86.'00. Leo R. Redner, Omak, Wash., April23, age 85.'01. Hamilton Nesbit Morrow, Fremont,Nebr., August 9, age 85.'02. Charles G. Davies, Blue Island, Ill.,April 15, age 85. .Ernst Starr Schmidt, Green Bay; Wis.,August 30, age 86.'03. William Robert Calderwood, SaltLake City, Utah, August 7; age 94.William Albert Clark, Jr., New Wil­mington, Pa., August 23, age 81.Findley D. John, Aberdeen, Miss., Sep­tember 11, age 89.'05. Galen A. Fox, Clearwater, Fla., Feb­ruary 17, age 81.James McBryde Knox, Cedar Rapids,Iowa, September 5, age 81.Ausby L. Lowe, Jr., Robinson, TIl., De­cember 19, age 80.'06. Ora G. Brubaker, North Manchester,Ind., January 28, age 86.Reuben W. Williams, Phoenix, Ariz.,January 25, age 80.'11. Philip Lewin, Chicago, May 13, 1960,age 72.S. Leah Riker Richelderfer, Wasco, Ore.,November 7, age 80.'17. Elmer R. Hancock, Chicago, Janu­ary 17, age 68. �- -, '20. Wallace Hugh Budge, Spokane,Wash., August 4, age 62.John Clark Hubbard, Price, Utah, Octo­ber 15, age 70.Julius Kahn, Beverly Hills, Calif., Feb.4, age 65.'22. Anton Hyden, Sioux Falls, S.D.,February 2, age 63.'23. Tell Nelson, Honolulu, November18, age 61.Edmound Lloyd Bernhardt, Milwaukee,Wis., August 11, age 63.Victor Josiah Hays, Chicago, November2, age 70.John V. Lawrence, St. Louis Mo., June12, age 67.'25. Guy Edwin Carlson, Berwyn, Ill.,January 3, age 69. -'26. Julius M. Amberson, Silver Springs,Md., February 18, age 63.'27. Arthur L. Higbee, Detroit, Mich.,June, age 59.Edith E. Norman, Peotone, 111., Decem­ber 5, age 70.'29. Herman Beuker, Marshall, Mich.,August 7, age 62.'32. Adelaide McFayden Johnson, Roch­ester, Minn., November 20, age 55.'33. Edward Theodore' Torwick, Spring­field, Mo., October 25, age 58.'34. Marion M. Morgan, Montgomery,Ala., January 28, age 63.'38. Richard Henry Callahan, East Chi­cago, Ind., December 16, age 46.'42. Lucius W. Wimby, Chicago, Janu­ary 8, age 43.'54. Agatha J. Sobel, Washington, D.C.,June 14, age 40.FACULTYRalph R. Ferguson, Faculty, '04-'09, St.Petersburg, Fla., October 9, age 82.Herman Neils Bundesen, Lecturer, '26,Chicago, August 25, age 78.